Researchers have developed gene-carrying nanoparticles that zero in on target cells and prevent vision loss in animal models of an inherited eye disease.
This inherited eye disease is also one of the most common causes of blindness in children.
Though this research focused on the form of the disease called Leber congenital amaurosis 2 (LCA2), the scientists and engineers involved in the study believe the technology holds promise for other forms of LCA as well as other inherited diseases that lead to severe vision loss or blindness.
Zheng-Rong Lu, a lead researcher and Professor at Case Western Reserve University in Cleveland, Ohio, US was quoted saying that “We believe this technology can deliver almost any type of gene to tackle inherited visual disorders.”
Those with LAC2 carry a mutated RPE65 gene and suffer from profound vision loss from birth.
The mutated gene fails to produce RPE65 protein in the retinal pigment epithelium (RPE), a cell layer critical for protecting photoreceptors (rods and cones).
The protein is an essential constituent of the visual cycle that converts light to electrical signals to the brain.
Lu and colleagues designed a lipid-based nanoparticle called ECO to deliver healthy RPE65 genes to RPE cells.
A study was conducted in which the retina of the mice was injected, the researchers could see fluorescent green concentrating in RPE cells.
Testing showed a significant increase in light-induced electrical activity from the eyes to the brain, indicating the rods and cones were operating as they should in the visual cycle.
Conclusions made at the end of the test were that the therapeutic effect lasted 120 days in treated mice and there were no improvements observed in the untreated mice.