The much-celebrated CRISPR/Cas9 gene editing technique can cause greater genetic damage in cells than was previously thought, scientists have warned.
CRISPR/Cas9 is a type of molecular scissor technology that can alter sections of DNA in cells by cutting at specific points and introducing changes at that location.
Besides extensive use in scientific research, CRISPR/Cas9 has also been seen as a promising way to create potential genome editing treatments for diseases such as HIV, cancer or sickle cell disease.
But the new research, reported in the journal Nature Biotechnology, revealed that CRISPR/Cas9 frequently caused extensive mutations, though at a distance from the target site.
Many of the cells, in both mice and humans, had large genetic rearrangements such as DNA deletions and insertions.
These could lead to important genes being switched on or off, which could have major implications for CRISPR/Cas9 use in therapies.
In addition, some of these changes were too far away from the target site to be seen with standard genotyping methods, the researchers said.
The work has implications for how CRISPR/Cas9 is used therapeutically and is likely to re-spark researchers’ interest in finding alternatives to the standard CRISPR/Cas9 method for gene editing.